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KMID : 0811720110150030143
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Korean Journal of Physiology & Pharmacology
2011 Volume.15 No. 3 p.143 ~ p.147
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The Relaxing Effect of α-Defensin 1 on the Adrenergic Responses of Rat Bladder
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Lee Shin-Young
Kim Don-Kyu
Kim Kyung-Do
Myung Soon-Chul
Lee Moo-Yeol
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Abstract
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Defensins, cysteine-rich cationic polypeptides released from neutrophils, are known to have powerful antimicrobial properties. In this study, we sacrificed 30 rats to investigate the effects of α-defensin 1 on detrusor muscle contractions in isolated rat bladder. From the experiments we found relaxing effects of α-defensin 1 on the contractions induced by phenylephrine (PE) but not by bethanechol (BCh) in the detrusor smooth muscles. To determine the mechanisms of the effects of α-defensin 1, the changes of effects on PE-induced contraction by α-defensin 1 pretreatment were observed after pretreatment of Rho kinase inhibitor (Y-27632), protein kinase C (PKC) inhibitor (Calphostin C), potent activator of PKC (PDBu; phorbol 12,13-dibutyrate), and NF-κB inhibitors (PDTC; pyrrolidinedithiocarbamate and sulfasalazine). The contractile responses of PE (10-9~10-4 M) were significantly decreased in some concentrations of α-defensin 1 (5×10-9 and 5×10-8 M). When strips were pretreated with NF-κB inhibitors (PDTC and sulfasalazine; 10-7~10-6 M), the relaxing responses by α-defensin 1 pretreatment were disappeared. The present study demonstrated that α-defensin 1 has relaxing effects on the contractions of rat detrusor muscles, through NF-κB pathway. Further studies in vivo are required to clarify whether α-defensin 1 might be clinically related with bladder dysfunction by inflammation process.
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KEYWORD
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α-Defensin, Detrusor smooth muscle, NF-κB, Adrenergic system
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